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Daltons to Kilodaltons Converter

↔ Convert kDa to Da instead

Common Conversions

Da kDa
100 0.1
500 0.5
1000 1
5000 5
10000 10
25000 25
50000 50
66500 66.5
100000 100
150000 150
500000 500
1000000 1000

Why this conversion matters in chemistry

Peptides come off a mass spectrometer in daltons — a tryptic fragment around 1500 Da, an intact small protein around 14,000. SDS-PAGE bands and size-exclusion chromatography apparent molecular weights live in kilodaltons — lysozyme at 14.3 kDa, BSA at 66.5, an IgG at 150. The conversion is dividing by 1000, but it earns its keep when a precision MS measurement of a peptide has to land in the same conversation as a gel-band annotation done at three significant figures. Same number, different scale, depending on which technique generated it.

Formula

kDa = Da / 1000

Worked Examples

66500 Da = 66.5 kDa

Bovine serum albumin (BSA) — the standard protein-chemistry molecular-weight reference.

1000 Da = 1 kDa

Roughly the mass of a small peptide — about nine residues, depending on composition.

150000 Da = 150 kDa

An intact IgG antibody — the size that gives a single band on a non-reducing SDS-PAGE gel.

25000 Da = 25 kDa

About the size of a small monomeric enzyme like chymotrypsin or trypsin (~23–25 kDa) — the kind that gives a clean single band at the lower end of an SDS-PAGE gel.

Frequently Asked Questions

How do I convert Da to kDa?
Divide by 1000. The relationship is exact, so 66,500 Da becomes precisely 66.5 kDa with no rounding.
Is a dalton the same as an amu?
Numerically, yes. Both are defined as 1/12 the mass of an unbound C-12 atom at rest in its electronic ground state. The dalton is the preferred name in biochemistry; the amu shows up more in physics and physical-chemistry contexts.
What's the kDa range for typical proteins?
Small folded domains and peptide-sized proteins run 5–25 kDa. Mid-size enzymes and many globular proteins fall in the 25–100 kDa range. Large multi-subunit complexes start at 100–500 kDa, and macromolecular assemblies (ribosomes, virus capsids) push into the megadalton range.