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Malic Acid

C4H6O5 organic

Properties

StateSolid (white crystalline powder with strong sour taste)
ColorWhite
SolubilityVery soluble in water (558 g/L at 20 °C); soluble in ethanol
Melting Point130 °C (L-form)
Boiling PointDecomposes before boiling

About Malic Acid

Malic acid is the alpha-hydroxy dicarboxylic acid you taste when you bite into a green apple. Carl Wilhelm Scheele crystallized it from apple juice in 1785 and named it from Latin malum (apple); the L-enantiomer dominates fruit (apples, grapes, cherries, rhubarb) and is the biologically active form, while the racemic DL synthetic material made from maleic anhydride hydration is what mostly shows up on industrial drum labels. The molecule does heavy lifting in two completely separate domains. In intermediary metabolism, L-malate is the Krebs cycle intermediate produced by fumarase from fumarate and oxidized by malate dehydrogenase to oxaloacetate (L-malate + NAD+ -> oxaloacetate + NADH + H+), pumping reducing equivalents into the electron transport chain. The malate-aspartate shuttle uses this same chemistry to ferry cytosolic NADH equivalents across the inner mitochondrial membrane during aerobic glycolysis, which is one reason why malate dehydrogenase deficiency is so quickly lethal. In the wine and food industry, malic acid is what makes Granny Smith apples mouth-puckering and what gets converted to softer L-lactic acid by Oenococcus oeni and Lactobacillus species during the malolactic fermentation that finishes red wines and big white Chardonnays. Winemakers either encourage MLF (most reds, oaked Chardonnay) or block it with SO2 and sterile filtration (Riesling, Sauvignon Blanc) depending on whether the goal is buttery roundness or crisp acidity. Sour-candy formulators love malic acid because its slow taste-release gives the long-lasting pucker that citric acid cannot produce.

Where you'll encounter it

If you have ever puckered through a Sour Patch Kid, tasted the buttery diacetyl note in an oaked Chardonnay that finished MLF, or pipetted L-malate into a coupled NADH-malate dehydrogenase enzyme assay, you have been working with malic acid. Candy formulators dust DL-malic acid on top of a sugar shell because the slow pH drop on the tongue holds the sour signal for 30 to 60 seconds, where citric flashes and fades inside 10. Winemakers running a Burgundy-style Chardonnay deliberately innoculate Oenococcus oeni after primary fermentation finishes to drop titratable acidity by a third and let diacetyl build for the buttery finish. In a biochem teaching lab, the L-malate plus NAD+ plus malate dehydrogenase reaction is the cleanest 340 nm absorbance jump a student will see all semester.

Common Uses

  • Sour-candy and fruit-beverage acidulant (E296), often blended with citric for taste-time profile
  • Substrate in malolactic fermentation in winemaking (L-malate -> L-lactate + CO2)
  • Krebs cycle intermediate in coupled NAD+/NADH enzyme assays
  • Alpha-hydroxy acid (AHA) chemical exfoliant in skincare at 1-10% concentration
  • Pharmaceutical salt former and chelator (e.g., trimebutine maleate, magnesium malate)
  • Plant-based meat-flavor system component (umami plus acid balance)

Safety Information

GRAS for food and supplement use. Concentrated solid and concentrated solutions can irritate eyes and broken skin (typical for any small organic diacid). 5 to 10% topical AHA formulations are well tolerated but increase UV sensitivity, so daily SPF is the standard recommendation. Oral doses up to several grams per day in supplement form are tolerated by most adults; very high intakes can produce GI cramping. Not classified hazardous under GHS at food-grade concentrations. Industrial DL-malic acid is chemically equivalent in safety profile to the L-form for food use.

This safety summary is for educational reference only and may not be complete. It is not a substitute for Safety Data Sheets (SDS), medical advice, or professional chemical safety guidance. Always consult appropriate SDS and qualified professionals before handling chemicals.

Constituent Elements

Frequently Asked Questions

What is the molar mass of malic acid?
Malic acid (C4H6O5) is 134.087 g/mol: 4 C (48.044) + 6 H (6.048) + 5 O (79.995). The two pKa values that matter for buffer work are pKa1 = 3.40 and pKa2 = 5.11, which puts useful buffering range at pH 2.5 to 6 — handy for fruit-beverage formulation work.
What role does malic acid play in the citric acid cycle?
Two roles. Step seven of the cycle is the hydration of fumarate to L-malate by fumarase. Step eight is the NAD+-dependent oxidation of L-malate to oxaloacetate by malate dehydrogenase, generating one NADH per turn that feeds Complex I of the electron transport chain. The oxaloacetate then condenses with the next acetyl-CoA from glycolysis to restart the cycle. Malate is also the carrier in the malate-aspartate shuttle that moves cytosolic NADH equivalents into the matrix in cells without an active glycerol-3-phosphate shuttle (notably heart and liver).
What is malolactic fermentation?
MLF is the bacterial conversion of sharp L-malic acid (diprotic, pKa 3.4) to softer L-lactic acid (monoprotic, pKa 3.86) plus CO2, performed by Oenococcus oeni or Lactobacillus species after primary alcoholic fermentation has finished. The net effect is a drop in titratable acidity by roughly a third, a small pH rise of 0.1 to 0.3 units, and a softer mouthfeel. The same bacteria produce diacetyl as a metabolic byproduct, which is the buttery aroma of oaked Chardonnay. Winemakers encourage MLF in nearly all reds and full-bodied whites, and block it with SO2 plus sterile filtration in aromatic varieties where bright acidity is the point.