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Potassium Acetate

KC2H3O2 salt

Properties

StateSolid (white crystalline, hygroscopic)
ColorWhite
SolubilityVery soluble in water (2530 g/L at 20 °C); soluble in methanol
Melting Point292 °C
Boiling PointDecomposes before boiling

About Potassium Acetate

Potassium acetate (KC2H3O2 or CH3COOK, 98.142 g/mol) is the potassium salt of acetic acid — a hygroscopic white powder that dissolves to about 2.5 kg per liter of water and gives a mildly basic solution because acetate is the conjugate base of a weak acid (Ka of acetic acid = 1.8 × 10^-5, so a 1 M acetate solution sits near pH 9.4). Two industries account for most of its tonnage. The first is airport runway de-icing: the FAA-approved Cryotech E36 product (50 percent KCH3COO solution) and several competing brands replaced glycol-based de-icers at most major airports because potassium acetate is biodegradable, non-corrosive to the aluminum alloys used in aircraft skins and landing gear, and effective down to about -26 °C. The second is molecular biology: the Birnboim-Doly alkaline lysis miniprep relies on a 5 M potassium acetate / glacial acetic acid solution at the neutralization step, where K+ co-precipitates with SDS, chromosomal DNA, and denatured protein, leaving plasmid DNA in solution. Smaller volumes go to food preservation and acidity regulation as E261, intravenous and oral potassium replacement in clinical hypokalemia (especially when chloride loading is contraindicated), buffer prep for chromatography, and as a catalyst in polyurethane foam manufacturing.

Where you'll encounter it

If you've ever run a plasmid miniprep, you've used potassium acetate — Solution III in the standard Qiagen/Macherey-Nagel kits is essentially 3 M potassium acetate at pH 5.5, and the snowy white precipitate that forms on the side of a 1.5 mL Eppendorf tube the moment you invert it is potassium dodecyl sulfate dragging chromosomal DNA and protein out of solution. If you've flown out of JFK, O'Hare, or Frankfurt in winter, the slippery wet film on the runway after a de-icing pass is a potassium acetate solution doing its work without eating the cadmium plating on landing gear. In the clinical lab, a potassium acetate IV piggyback is the alternative to KCl for a hypokalemic patient with metabolic alkalosis where adding more chloride would make things worse — the acetate metabolizes to bicarbonate and helps correct the acid-base imbalance at the same time it replenishes potassium.

Common Uses

  • FAA-approved airport runway de-icer (Cryotech E36 and equivalents), non-corrosive to aluminum alloys
  • Solution III in Birnboim-Doly alkaline-lysis plasmid DNA miniprep (3-5 M, pH 5.5)
  • Food preservative and acidity regulator E261 in pickled and cured products
  • IV potassium replacement for hypokalemia with metabolic alkalosis (chloride-sparing alternative to KCl)
  • Acetate buffer component for HPLC and protein chromatography near pH 4-5
  • Catalyst for polyurethane foam manufacturing and isocyanate trimerization
  • Drying agent for ethanol via formation of the trihydrate
  • Fire-extinguishing agent in Class K wet-chemical kitchen extinguishers

Safety Information

Low acute toxicity (oral LD50 rat ~3250 mg/kg). Mild eye irritant (GHS H319). OSHA: no specific PEL — handle as a general chemical dust under the 15 mg/m3 total nuisance-dust limit. Not classified as flammable, carcinogenic, or environmentally hazardous. Hygroscopic, so store in tight containers; concentrated solutions are mildly basic and can sting cuts but pose no serious chemical hazard. Standard lab PPE — safety glasses, gloves, lab coat — is sufficient.

This safety summary is for educational reference only and may not be complete. It is not a substitute for Safety Data Sheets (SDS), medical advice, or professional chemical safety guidance. Always consult appropriate SDS and qualified professionals before handling chemicals.

Constituent Elements

Frequently Asked Questions

What is the molar mass of potassium acetate?
Potassium acetate (CH3COOK) has a molar mass of 98.142 g/mol, summed from one potassium (39.098), two carbons (2 x 12.011 = 24.022), three hydrogens (3 x 1.008 = 3.024), and two oxygens (2 x 15.999 = 31.998). The trihydrate KCH3COO 3H2O comes in at 152.19 g/mol, which is the form some bench reagent suppliers ship — always check the label before weighing for a stoichiometric prep.
Why is potassium acetate used for airport de-icing?
It satisfies four constraints that knock out most alternatives: it is non-corrosive to the aluminum-magnesium alloys in aircraft skins and landing gear, it is biodegradable and avoids the BOD spike that glycol de-icers create in airport runoff, it works down to about -26 °C, and the FAA has an established approval pathway for it (AMS 1435). Sodium chloride pits aluminum, glycols overwhelm sewage plants, and urea oxidizes too slowly — potassium acetate sits in the gap.
How is potassium acetate used in DNA extraction?
In the Birnboim-Doly alkaline-lysis miniprep, cells are lysed with NaOH/SDS (Solution II), then the lysate is neutralized with cold 3-5 M potassium acetate at pH 5.5 (Solution III). Two things happen at once: the pH drop lets supercoiled plasmid DNA snap back into duplex while denatured chromosomal DNA stays single-stranded and tangled, and potassium ions swap with sodium on the dodecyl sulfate to form insoluble potassium dodecyl sulfate, which precipitates and drags the chromosomal DNA, denatured protein, and cell debris out of solution. The supernatant carries clean plasmid DNA into the next column or precipitation step.