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Glycerol

C3H8O3 organic

Properties

StateLiquid at room temperature (viscous)
ColorColorless
SolubilityMiscible with water
Melting Point17.8 °C
Boiling Point290 °C

About Glycerol

Glycerol is the three-carbon triol HOCH2-CHOH-CH2OH — molar mass 92.094 g/mol, viscosity around 1500 cP at 20 °C (about 1500× water), boils at 290 °C, and remains liquid down to 17.8 °C with serious supercooling tendencies that let it stay fluid well below freezing. Three hydroxyls per molecule mean dense intermolecular hydrogen bonding, which is why glycerol is hygroscopic enough to pull moisture out of dry air, miscible with water in any ratio, and so viscous it pours like honey. It's the alcohol backbone of every triglyceride in every fat and oil, so when you saponify tallow with NaOH to make soap, or transesterify rapeseed oil with methanol to make biodiesel, glycerol comes out as the byproduct — about 100,000 tonnes a year as biodiesel waste-stream alone, which has driven the price down enough that glycerol is now economically competitive as a feedstock for downstream chemistry like acrolein, propylene glycol, and epichlorohydrin synthesis. The most chemically interesting thing about glycerol is what happens when you nitrate it: dropwise addition to a 1:1 HNO3/H2SO4 mixed acid below 30 °C esterifies all three hydroxyls to give nitroglycerin, the shock-sensitive oily explosive that Nobel adsorbed onto kieselguhr to invent dynamite (and which cardiologists still prescribe sublingually because vascular endothelium reduces it to NO, dilating coronary arteries within seconds).

Where you'll encounter it

If you've ever rubbed lotion into dry skin in winter, vaped an e-liquid (the 'VG' on the bottle is vegetable glycerin), watched a pharmacist hand a heart patient a tiny bottle of nitroglycerin tablets, or smelled the sweet note in the steam off freshly washed dishes, you've used glycerol — it pulls water from the air to your skin, vaporizes cleanly in e-cig coils at 200-250 °C, nitrates to a coronary vasodilator, and is what makes commercial dishwashing detergent feel slippery.

Common Uses

  • Humectant and viscosity modifier in skincare and toothpaste (E422)
  • Base liquid in e-cigarette and vape formulations (vegetable glycerin)
  • Cryoprotectant for sperm, embryo, and bacterial culture freezing (15% v/v final)
  • Sweetener and moisture retainer in soft-textured pet treats and chewy candy
  • Feedstock for nitroglycerin (dynamite, smokeless powder, vasodilator drug)
  • Sublingual base for nitroglycerin tablets in angina pectoris treatment
  • Lubricant and antifreeze in cold-weather hydraulic systems

Safety Information

GRAS for food, pharmaceutical, and cosmetic use; oral LD50 in rats is 12.6 g/kg, putting it in the 'practically nontoxic' bracket. No GHS hazard classification at typical concentrations. Real concerns are narrow: large oral doses (>1 g/kg) cause osmotic diarrhea because glycerol is poorly absorbed and pulls water into the gut lumen — this is why glycerin suppositories work as laxatives. Glycerol can be polyol-fermented by some gut bacteria into 3-hydroxypropionaldehyde (reuterin), which is irritating in concentrated form. Industrial-scale handling is unremarkable: not flammable below 160 °C flash point, not corrosive, no respiratory hazard. Vaping glycerol generates trace formaldehyde and acrolein when coil temperature exceeds 270 °C, which is the basis for low-power vape recommendations.

This safety summary is for educational reference only and may not be complete. It is not a substitute for Safety Data Sheets (SDS), medical advice, or professional chemical safety guidance. Always consult appropriate SDS and qualified professionals before handling chemicals. We deliberately do not publish occupational exposure limits or other regulatory thresholds: those values are revised over time and differ between jurisdictions, so the only correct source is the current SDS and the regulations that apply where you work.

Constituent Elements

Frequently Asked Questions

What is the molar mass of glycerol?
Glycerol (C3H8O3) weighs 92.094 g/mol: 3 carbons (36.033), 8 hydrogens (8.064), 3 oxygens (47.997). USP-grade anhydrous glycerin is 99.5%+ pure with the balance being water; food-grade is typically 99.7%. Density is 1.261 g/mL at 20 °C, so 100 mL of glycerin weighs about 126 g — useful when you're preparing 30% glycerol stocks for bacterial freezer aliquots and need to convert between volume and mass quickly.
Where does industrial glycerol come from?
Two main sources, in roughly equal volumes: saponification (alkaline hydrolysis of animal fats and vegetable oils for soap manufacture) and transesterification (oils plus methanol with NaOMe catalyst for biodiesel). Both reactions cleave all three ester bonds of a triglyceride and free the glycerol backbone. The biodiesel boom of the 2000s flooded the market with crude glycerol — at one point it was selling for under $0.10/lb — which kicked off serious research into glycerol-as-feedstock chemistry: acrolein, 1,3-propanediol, propylene glycol, dihydroxyacetone, glyceric acid, all now produced from glycerol. There's also a smaller fermentation route via yeast under sulfite stress (the original WWI route).
Why is glycerol used to freeze bacterial cultures?
Slow ice crystallization is what kills cells during freezing — sharp ice crystals shred membranes. Glycerol enters cells freely (it's small and uncharged), competes for water hydrogen-bonding sites, and vitrifies along with intracellular water rather than letting clean ice nucleate. The standard recipe is 15-25% glycerol final concentration mixed with overnight bacterial culture, then dropped into a -80 °C freezer; viable counts after a year typically drop less than 10×. The same trick works for human sperm (10% glycerol), oocytes (with sucrose as a co-CPA), and some mammalian cell lines (where DMSO often substitutes for glycerol because it permeates faster).